25th European Nitrogen Cycle Meeting

25th European
Nitrogen Cycle Meeting

From molecules to climate change

From molecules to climate change

Rome, Italy • 28-30 September 2022

Rome, Italy
28-30 September 2022


Day 1 Venue: Nobile Collegio Chimico Farmaceutico Via in Miranda 10 - Roma

28th September 2022

15:30-16:30 Welcome address and Congress Opening

15:45 Opening Lecture (35’ + 10’ Q&A)

T1 DAVID J. RICHARDSON UEA Norwich (UK) Bridging past, present and future of the European research on the Nitrogen Cycle (Sponsored by FEMS)

16:30 – 18:20 S1 - Impact of anthropogenic activities on N-cycle
Chairs: Conrado Moreno Viviàn (ES) – Elisabeth Baggs (UK)

16:30 Selected talks (8’ + 2’ Q&A)

T2 LOUISE SENNETT NMBU Ås (NO) The effects of chemical fumigation and biofumigation on the soil nitrogen cycle, abundance of nitrogen cycling microorganisms, and bacterial diversity (ECS-FEMS)

T3 MARIA J TORRES UCO Cordoba (ES) Nitrogen removal, increased algal biomass and hydrogen production are enhanced in Chlamydomonas/pink-pigmented bacterium co-cultures

T4 SHUAIMIN CHEN Jilin Academy of Agricultural Sciences (CN) Denitrification in the deep vadose zone

T5 MIGUEL SEMEDO CIIMAR Matosinhos (PT) Cadmium effects on net N2O production by a denitrifying deep-sea isolate

17:10 Flash poster presentation (2’ each)

P1 AVSAR CUMHUR NMBU Ås (NO) Mechanisms controlling denitrification under feast and famine (ECS-FEMS)

P2 LUKAS GEISENHOF Darmstadt University (DE) Characterization of Neobacillus vireti laughing gas respiration (ECS-FEMS)

P3 TIMON LAADER Darmstadt University (DE) Isolation and characterization of laughing gas reducing bacteria from various environments (ECS-FEMS)

P4 INETA LIEPIŅA-LEIMANE Latvian Institute of Aquatic Ecology, DU Rīga (LV) Seasonal pattern and regulating factors of diazotrophic activity in the Gulf of Riga Skultes port, Baltic Sea

P5 PETRA PJEVAC University of Vienna (AT) Same, same but, different – distinct metabolic roles of NXR homologues in Nitrospira moscoviensis

P6 CARMEN MARIA BELLIDO PEDRAZA UCO Cordoba (ES) The nitrite reductase mutants reveal the importance of nitrate reductase in N2O production in Chlamydomonas reinhardtii

P7 DAVID BURTON Dalhousie University, Halifax (CA) Reduced Rate Is Key to Realizing N2O Emission Reduction from Enhanced Efficiency Fertilizers

P8 MARINA SMILEY Columbia, NY (US) Cross-Talk Between Denitrification And Phenazine Production In Pseudomonas Aeruginosa

P9 KELLY ECKARTT Columbia, NY (US) Light Affects Denitrification in Pseudomonas Aeruginosa Biofilms

P10 TOMAS LEIGH UEA Norwich (UK) sRNA-11 regulates the growth of the bacterial denitrifier Paracoccus denitrificans

P11 BRAM VEKEMAN Max Planck Institute for Marine Microbiology, Bremen (DE) Versatile anaerobic ammonium-oxidizing bacteria can use alternative carbon and nitrogen sources for growth and energy conservation

17:35 Keynote lecture (35’ + 10’ Q&A)

T6 DAVID FOWLER UK-CEH Edinburgh (UK) Human activity and the nitrogen cycle (Sponsored by FEMS)

18:20 Wine reception (venue rooftop)

Day 2-3 Venue: Aula Gerin - Medicina Legale (building CU023), Sapienza University Via C. De Lollis 23 - Roma

29th September 2022

9:00-11:00: S2 Denitrification
Chairs: Serena Rinaldo (I) – Alessandro Giuffrè (I)

9:00 Keynote lecture (35’ + 10’ Q&A)

T7 GARY ROWLEY UEA Norwich (UK) Uncovering the regulatory sRNA controlling bacterial denitrification and N2O production (Sponsored by FEMS)

9:45 Selected talks (8’ + 2’ Q&A)

T8 JOSE MARÍA MIRALLES ROBLEDILLO University of Alicante (ES) Overexpression, purification, and analysis of the role of a dtxr-type metal-dependent regulator in haloarchaeal denitrification (ECS-FEMS)

T9 MARTIN MENESTRAU NMBU Ås (NO) Diverse denitrification regulatory phenotypes among strains of Pseudomonas stutzeri

T10 CARMEN PIRE University of Alicante (ES) Analysis of transcriptome changes associated with denitrification in Haloferax mediterranei

T11 SOPHIE MOELLER UEA Norwich (UK) Small RNA control of denitrification and nitrous oxide emissions in bacteria (ECS-FEMS)

10:25 Invited speaker (15’ + 5’ Q&A)

T12 MARÍA DOLORES ROLDÁN RUIZ UCO Cordoba (ES) Exploring denitrification through proteomics

10:45-11:15 Coffee break – Poster session

11:15-12:45 S3: N2O emission
Chairs: Lars Dietrich (US) – Francesca Cutruzzolà (I)

11:15–11:20 Presentation of the Microbiology Society


11:20 Keynote lecture (35’ + 10’ Q&A)

T13 ÅSA FROSTEGÅRD NMBU Ås (NO) Bacterial control of N2O emissions – new mechanisms, organisms and potential applications (Sponsored by Microbiology Society)

12:05 Selected talks (8’ + 2’ Q&A)

T14 KJELL RUNE JONASSEN NMBU Ås (NO) Biotechnology for reducing N2O emission from soil: dual enrichment for isolating the best N2O-reducing bacteria

T15 ELISABETH GAUTEFALL HIIS NMBU Ås (NO) Manipulating soil metabolism for reduced N2O-emissions: a niche for N2O-reducing bacteria ex situ

T16 KRISTINE RØSDAL NMBU Ås (NO) The phenotype of Cloacibacter, a superb N2O sink

12:35 Invited speaker (15’ + 5’ Q&A)

T17 MARIA J DELGADO EEZ-CSIC, Granada (ES) Nitric oxide and nitrous oxide emissions by plant-associated endosymbiotic bacteria: processes involved and regulatory network

12:55-14:15 Lunch – Poster session

14:15-14:55 S4: N2O reduction and respiration
Chairs: Maria J. Delgado (ES) - Åsa Frostegård (NO)

14:15 Selected talks (8’ + 2’ Q&A)

T18 LIN ZHANG Albert Ludwig University of Freiburg (DE) Mechanistic insights into the biogenesis and regulation of nitrous oxide reductase

T19 PAWEL LYCUS NMBU Ås (NO) Souring N2O emissions

T20 NINA ROOTHANS NMBU Ås (NO) Long-term ecophysiological profiling of the wastewater microbiome underlying N2O emissions

T21 SUKHWAN YOON Korea Advanced Institute of Science and Technology Yuseong-gu (KR) N2O inhibition of DNRA activity in a nrfA-possessing soil isolate Bacillus sp. DNRA2 implying a new function of nitrous oxide reductase in DNRA-catalyzing microorganisms

14:55-15:10 A novel European Infrastructure Network on Molecular Biophysics (MOSBRI)


15:10–15:40 Coffee break – Poster session

15:40-16:30 S5: NH4+: from cell to soil
Chair: Víctor Manuel Luque-Almagro (ES)

15:40 Invited speaker (15’ + 5’ Q&A)

T23 ELISABETH BAGGS University of Edinburgh (UK) A plant-trait approach for enhancing soil N processes

16:00 Selected talks (8’ + 2’ Q&A)

T24 CHRIS SEDLACECK University of Vienna (AT) Cellular physiology and kinetics govern the substrate competitiveness of ammonia oxidizers

T25 ELOI MARTINEZ-RABERT University of Glasgow (UK) Competitive and substrate limited environments drive metabolic diversity for comammox nitrospira

T26 FRANCESC CORBERA RUBIO TU Delft (NL) Nitrification moves: stratification patterns in rapid sand filters for drinking water production

18:00 Baroque Rome walking tour and Social dinner at “Al Pompiere” (20:00)

30th September 2022

9:00–10:25 S6: Biosignaling and communication
Chairs: David Richardson (UK) – Rosa Maria Martinez-Espinoza (ES)

9:00 Keynote lecture (35’ + 10’ Q&A)

T27 DAVID LILLEY University of Dundee (UK) The recognition of small metabolite molecules by RNA in genetic control by the riboswitches (Sponsored by Microbiology Society)

9:45 Selected talks (8’ + 2’ Q&A)

T28 RICARDA KELLERMANN NMBU Ås (NO) Quorum sensing restricts N2O reduction in Pseudomonas aeruginosa and Pseudomonas fluorescens F1133 (ECS-FEMS)

T29 ANDREW GATES UEA Norwich (UK) Control of bacterial nitrate assimilation and nitrous oxide respiration by DNA G-quadruplex structures

10:05 Invited speaker (15’ + 5’ Q&A)

T30 SERENA RINALDO Sapienza University of Rome (IT) Member of the Italian Society of Microbiology Linking L-arginine and redox sensing in Pseudomonas aeruginosa to control c-di-GMP levels and biofilm formation

10:25–11:05 S7: Linking Nitrogen to Carbon and Sulphur metabolism
Chairs: Alessandro Giuffrè (I) – Lars Bakken (NO)

10:25 Selected talks (8’ + 2’ Q&A)

T31 ELISA MERZ Max Planck Institute for Marine Microbiology, Bremen (DE) Bentic diatoms from a microbial mat couple nitrate reduction to sulphide oxidation

T32 STEPHEN SPIRO University of Texas at Dallas (USA) The pathway for choline catabolism in Paracoccus denitrificans - identification of the source of formaldehyde

T33 FRANCESCA GIORDANO Sapienza University of Rome (I) Persulfide Dioxygenase (PDO) from Pseudomonas aeruginosa at the crossroad of hydrogen sulfide and nitric oxide (ECS FEMS)

T34 ROB VAN SPANNING Vrije Universiteit Amsterdam (NL) A methanotrophic Mycobacterium dominates a cave microbial ecosystem

11:05-11:35 Coffee break – Poster session

11:35–12:45 S8: Bacterial respiration and N2 metabolism: variations on the theme
Chair: Serena Rinaldo (I) – Gary Rowley (UK)

11:35 Invited speaker (15’ + 5’ Q&A)

T35 LARS DIETRICH Columbia NY (USA) The interplay of respiration and denitrification in Pseudomonas aeruginosa biofilms

11:55 Selected talks (8’ + 2’ Q&A)

T36 MARTINA ROBERTA NASTASI Sapienza University of Rome (I) The Pseudomonas aeruginosa cyanide insensitive oxidase sustains sulfide-resistant respiration and nitric oxide resistance (ECS FEMS)

T37 CHIARA SCRIBANI ROSSI Sapienza University of Rome (I) Effect of l-arginine on P. putida energy metabolism: role of arginine sensors in the metabolic reprogramming (ECS FEMS)

T38 LINDA BERGAUST NMBU Ås (NO) Facing anoxia; the roles of O2 and N2O in the bet-hedger Paracoccus denitrificans switching from aerobic respiration to denitrification

12:25 Invited speaker (15’ + 5’ Q&A)

T39 ALESSANDRO GIUFFRÈ IBPM-CNR (I) NO and bacterial respiratory oxidases

12:45-14:10 Lunch – Poster session

14:10-15:20 S9: Impact of N-cycle on biotechnology
Chairs: Åsa Frostegård (NO) - Francesca Cutruzzolà (I)

14:10 Invited speaker (15’ + 5’ Q&A)

T40 VÍCTOR MANUEL LUQUE-ALMAGRO UCO Cordoba (ES) Cyanide as a Nitrogen Source: An Overview of Bacterial Cyanide Biodegradation

14:30 Selected talks (8’ + 2’ Q&A)

T41 PALOMA GARRIDO AMADOR Max Planck Institute for Marine Microbiology, Bremen (DE) Breathing poison: enrichment of a nitric oxide-reducing microbial community in a continuous bioreactor

T42 MICHELE LAURENI TU Delft (NL) Affinity-based enrichment of clade II N2O-reducers

T43 MARTE MAARAK NMBU Ås (NO) Anaerobic high cell density cultivation by denitrification: potential and challenges

15:00 Invited speaker (15’ + 5’ Q&A)

T44 ROSA MARIA MARTINEZ-ESPINOZA University of Alicante (ES) Denitrifying haloarchaea: a powerful tool for bioremediation

15:20 – 15:50 Coffee break – Poster session

15:50–16:50 S10 Closing session
Chair: David Richardson (UK)

15:50 Keynote lecture (35’ + 10’ Q&A)

T45 NIKHIL MALVANKAR Yale University, New Haven (US) Observation of single Geobacter cells transferring electrons via cytochrome nanowires secreted by pili

16:35 Concluding remarks